Insertion junction: CLIP2.037185_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.037185
Chromosome:chromosome 14
Location:866598
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g613100 (1 of 1) IPR008999 - Actin cross-linking intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGGTAAACACCAACCGGGGGTCTGGGGGGTGTCCCAGCCGGGGGGCCG
Internal bar code:CGCCAGGACGCAAGTCACGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3294
LEAP-Seq percent confirming:98.3333
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTGCATGTCGTGAGTCTT
Suggested primer 2:GACGTCAACCCAAAGTGTGC