Insertion junction: CLIP2.037186_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.037186
Chromosome:chromosome 16
Location:5057000
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g685901 (1 of 1) K18423 - exportin-2 (importin alpha re-exporter) (CSE1, CAS, XPO2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACATCCAATGCCCCCGTCCCCACGCTATAACAAACGTCGCGCCAGCTC
Internal bar code:TGGTCTTTCTTAACCTTATATT

Confirmation - LEAP-Seq

LEAP-Seq distance:153
LEAP-Seq percent confirming:52.9412
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGGTTGTCATTCAGCTTG
Suggested primer 2:GTTTGGTTGCGCTGGGTATC