| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037194 |
| Chromosome: | chromosome 1 |
| Location: | 4283441 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028950 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGCACCGACGGATAGACTCCGTCGCCGCGTCTGCAGCAAGCAGGGCC |
| Internal bar code: | ACTAAATTTGTTTGGATTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 8.69565 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGTGATGTTCTGGGCGC |
| Suggested primer 2: | AGTCGCTCAGGAGGCTAAGA |