| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037271 |
| Chromosome: | chromosome 1 |
| Location: | 4382898 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029800 | Coiled-Coil Protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCAGCAGCACTTCCGCAACCGCGAGGACATTCGCGGCAAGGTGGTCC |
| Internal bar code: | TCACAAATTTGTTGGAGCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5240 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAATCGGATGTCACCTGC |
| Suggested primer 2: | CATGTATGCACGCGTTGGAG |