Insertion junction: CLIP2.037276_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.037276
Chromosome:chromosome 3
Location:536175
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145187 (1 of 274) IPR020683 - Ankyrin repeat-containing domain 3'UTR
Cre03.g800287 (1 of 1) PF00665//PF13976 - Integrase core domain (rve) // GAG-pre-integrase domain (gag_pre-integrs) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGACCACTCTGTCTGCGCTGCGTGCTTGCGCTGCCGCCGGCGCTGCGAC
Internal bar code:GCCTAACAATCCAGTGTAGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2004
LEAP-Seq percent confirming:94.4444
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGACTATGCTGGAGGCTG
Suggested primer 2:GTATACGGGGCAGACGTCAG