| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037276 |
| Chromosome: | chromosome 3 |
| Location: | 536178 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145187 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 3'UTR | |
| Cre03.g800287 | (1 of 1) PF00665//PF13976 - Integrase core domain (rve) // GAG-pre-integrase domain (gag_pre-integrs) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTTCTCAGCGCTAGCAGATCCTTTAAGCCAATAATAGAGCCGGTACC |
| Internal bar code: | GCCTAACAATCCAGTGTAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2752 |
| LEAP-Seq percent confirming: | 89.5349 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATACGGGGCAGACGTCAG |
| Suggested primer 2: | CGAGACTATGCTGGAGGCTG |