| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037291 |
| Chromosome: | chromosome 4 |
| Location: | 692995 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217953 | (1 of 2) PTHR15241:SF0 - TRANSFORMER-2 SEX-DETERMINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCGGTGGTGGCGGCGGCAGCAGCTGGGGCAGGTGTGGCAGCGGGGAC |
| Internal bar code: | GGGCCAAGGGGGCGACGGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3449 |
| LEAP-Seq percent confirming: | 80.7018 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCAGCTCACTTGACAAAC |
| Suggested primer 2: | CGCAGGTGGGCATTTTTGAA |