Insertion junction: CLIP2.037295_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.037295
Chromosome:chromosome 16
Location:4896008
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686950 CTF18 DNA damage repair and chromosome cohesion protein; (1 of 1) K11269 - chromosome transmission fidelity protein 18 (CTF18, CHL12) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGACACGCTGCACTGCTTCACGGCATTCGCGGGCGGCGCGGCGGCGGGC
Internal bar code:TTATAGGTTCTACCTGTTTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3891
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GAACGCATCCATCCATGTGC
Suggested primer 2:CGACGCTGATGCTGTTGATG