Insertion junction: CLIP2.037295_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.037295
Chromosome:chromosome 16
Location:4896008
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686950 CTF18 DNA damage repair and chromosome cohesion protein; (1 of 1) K11269 - chromosome transmission fidelity protein 18 (CTF18, CHL12) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCCGGCACCAGCGGCATGGCGCGCACCGCAGCTGCCGCGGCCGCGGCC
Internal bar code:TTATAGGTTCTACCTGTTTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:116
LEAP-Seq percent confirming:76.9231
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CGACGCTGATGCTGTTGATG
Suggested primer 2:GAACGCATCCATCCATGTGC