| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.037296 |
| Chromosome: | chromosome 12 |
| Location: | 731695 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492750 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGTCAGCCTCGCGCTTGGCCACGGCCGCCTGCTGCTGACGCGCTGCG |
| Internal bar code: | GTGGTTATTTGAAGCCTTTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1310 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTTGCCTTCCAATAGGT |
| Suggested primer 2: | AGTGCCTACCTCGTCCATCT |