Insertion junction: CLIP2.037312_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.037312
Chromosome:chromosome 5
Location:2548851
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g239400 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTTTCTTATTGAACTCGCTACGCAGCGTGCGCAGTTCCGTCAGAAGCT
Internal bar code:TGACAATAGCTCACGCACAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:989
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACGTAATTGCGAGGCGAAG
Suggested primer 2:CCATGACGACTGATGCTGGT