| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.037317 |
| Chromosome: | chromosome 3 |
| Location: | 2002833 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155800 | (1 of 1) K15025 - probable RNA-binding protein EIF1AD (EIF1AD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTCGGCGGACATTCGTGTATGTGGTTACAAGATGGTGCGCACAACCT |
| Internal bar code: | GAACTCGAAAATGGGTGAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2424 |
| LEAP-Seq percent confirming: | 72.1311 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTAGCCGCTCCTCACATC |
| Suggested primer 2: | GTGGGTTGTGTGTTGGATGC |