Insertion junction: CLIP2.037338_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.037338
Chromosome:chromosome 9
Location:5822007
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g411500 URN4 (1 of 1) K01239 - purine nucleosidase (iunH); Putative inosine/uridine-preferring nucleoside hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGTGGGTGGAATGACGAAAGGTTATGCCGCGGTACCCCATCAAGTTTC
Internal bar code:TTCACGTTTGGTTCAAGACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:381
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGTCCTAGCACTCGATGT
Suggested primer 2:GTCATCGGCATCACCATCCT