| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037360 |
| Chromosome: | chromosome 10 |
| Location: | 1786112 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430700 | FAP308 | Flagellar Associated Protein 308 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACACACACACACACACACACACACACACACACACACACACAC |
| Internal bar code: | GCTACGCTGACAAATCCGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1581 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGATGCGTAAGACGAGG |
| Suggested primer 2: | CTTACCTCAACCCCCACACC |