| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037375 |
| Chromosome: | chromosome 3 |
| Location: | 4318773 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174400 | CDO1 | Cysteine dioxygenase, regulated by copper nutrition; (1 of 2) 1.13.11.20 - Cysteine dioxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCACGTAGTGGATGTCGTTGAACATGGCGTAGCGTCGCCAGTCGCCCG |
| Internal bar code: | TAGGCCTGTATCATGTTCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4412 |
| LEAP-Seq percent confirming: | 95.7895 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAACCCAGTCGCTTCAGG |
| Suggested primer 2: | GAAGGGAAGGGAAGGTCAGC |