Insertion junction: CLIP2.037378_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.037378
Chromosome:chromosome 3
Location:1115376
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g148750 CLH1 Chlorophyllase I; (1 of 1) PTHR33428:SF2 - CHLOROPHYLLASE-1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATAACGACGTCCAACGGAACCGCCATCGCAGAATCCACCCGCAACGTCA
Internal bar code:ACGCGTATTGCCTACTCATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2591
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCGGGAATTGAGGGTTCA
Suggested primer 2:GTGTACTGCACCACCGTGTA