| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.037382 |
| Chromosome: | chromosome 9 |
| Location: | 3261830 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394917 | (1 of 4) PTHR16255//PTHR16255:SF4 - UNCHARACTERIZED // SPORULATION PROTEIN RMD8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATCGCGTACCGTGCCGGGTGGGTAGGTGAGCCTCAGGAGCTCTTCCAG |
| Internal bar code: | GATACCCGAAGATTGGCACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3152 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGTTCATCACTGTGCCT |
| Suggested primer 2: | GCGAGAACACACACACACAC |