| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037392 |
| Chromosome: | chromosome 12 |
| Location: | 7054130 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560350 | CNK2 | (1 of 1) PTHR24361//PTHR24361:SF410 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED; NIMA-Related Kinase 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGCCGCCGGCGCCGTCCGCCGCGGCGGGCGGCAAGCTGCCGCAGCTG |
| Internal bar code: | GTTCTTTACGCCGACGCGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1505 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGAGAGGACCCCGACAGA |
| Suggested primer 2: | CATCCCCAACACCTTCTCCC |