| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.037422 |
| Chromosome: | chromosome 8 |
| Location: | 3555286 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379350 | TPT10,TPT | phosphate/phosphoenolpyruvate translocator; (1 of 2) PTHR11132:SF70 - PHOSPHOENOLPYRUVATE/PHOSPHATE TRANSLOCATOR 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCATTGGGCTTCGGCACGCGGCACCTACGCCCTGCTAACCACCTCCT |
| Internal bar code: | ATCACGCCATGGCATCTAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGCTTCCAGCCCTCAAA |
| Suggested primer 2: | TGTTGCCCTACCCGTTGTTT |