Insertion junction: CLIP2.037437_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.037437
Chromosome:chromosome 17
Location:5258041
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g802106 (1 of 32) IPR029062 - Class I glutamine amidotransferase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACACTGGCACGGGCGCGTGTGATTGTGTTTAGCACGATCTTCAGGTCA
Internal bar code:GACCGTACGTCGACGCCGAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1823
LEAP-Seq percent confirming:42.6966
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:51
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTTTAAGCACGCCTTCCT
Suggested primer 2:CGGCGTGCTCAGTTTTCAAA