| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037446 |
| Chromosome: | chromosome 12 |
| Location: | 5334040 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528614 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACCGTTGGAAGGGACGCTGACTGCCAGCCGGATCCGCGGATTTGAGC |
| Internal bar code: | CAGTTCGGCACATAAATAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4906 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 135 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 138 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACCTGTCAACAAAGCAG |
| Suggested primer 2: | CGCCCTTAATCATGCACACG |