| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037451 |
| Chromosome: | chromosome 7 |
| Location: | 5388002 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350450 | (1 of 2) PTHR22763:SF71 - PROTEIN Y105C5B.11, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAGGCCAAGCGCTACAAGCTGCACGTGGAGGCGCTGCTCGCGGAGATG |
| Internal bar code: | GAAACCCGCACGTCCTTTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3265 |
| LEAP-Seq percent confirming: | 45.3125 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTGCGGTTGTTGAGTTG |
| Suggested primer 2: | GCACGTGTTTCCACCAGAAC |