Insertion junction: CLIP2.037451_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.037451
Chromosome:chromosome 7
Location:5388007
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g350450 (1 of 2) PTHR22763:SF71 - PROTEIN Y105C5B.11, ISOFORM B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGCCTCTCGCTCCTGTGCCAGCCGCTGCGCCTGGCGGGCGTCCTGTG
Internal bar code:GTCGAAGTACAGACGATTTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2254
LEAP-Seq percent confirming:98.0392
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGTGTTTCCACCAGAAC
Suggested primer 2:CGACTGCGGTTGTTGAGTTG