| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037466 |
| Chromosome: | chromosome 12 |
| Location: | 5298777 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528200 | (1 of 1) K10742 - DNA replication ATP-dependent helicase Dna2 (DNA2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTCGTGTGCCATGCCTTTGGGCATTAGGAGCACCGGGCTCGCCGACG |
| Internal bar code: | AACGCCTCGGACGGTTGATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4070 |
| LEAP-Seq percent confirming: | 84.8485 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGATAGCGGTCGATGGTC |
| Suggested primer 2: | CGTCCGTCATGACCAATTGC |