| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.037481 |
| Chromosome: | chromosome 3 |
| Location: | 277632 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144404 | (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACACAACACACAATACGGTACACAACCCACGCACAGTCTGCCCTGGC |
| Internal bar code: | GGCTCGTTATTATGTTTAATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAATGGGATGTGTGGAG |
| Suggested primer 2: | CCGAGGCTCGTAAAACACCT |