Insertion junction: CLIP2.037494_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.037494
Chromosome:chromosome 8
Location:458331
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g358900 (1 of 3) 2.4.2.8 - Hypoxanthine phosphoribosyltransferase / Transphosphoribosidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCTGCGAGGTCGGCGGGACGGGTTGGCTCAGGCTGCGCGCCGGCGGT
Internal bar code:ATTGTCGTCGGCCACGTTATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1334
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TATCTAAGCTTGCGCGAGGG
Suggested primer 2:CACAAAACCCTTTCCCGCTG