| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037497 |
| Chromosome: | chromosome 3 |
| Location: | 6734567 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196200 | (1 of 2) PTHR17630//PTHR17630:SF32 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCAACGCTGCGGGTGTGCCGAACAGGCTGGAGCGGTTCGAGGGCGAG |
| Internal bar code: | GTATGTGTGTTAGAAAGGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3970 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCATCATCAACCGGCGG |
| Suggested primer 2: | GTGGACTCGGATCGCATCAT |