| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037497 |
| Chromosome: | chromosome 3 |
| Location: | 6734569 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196200 | (1 of 2) PTHR17630//PTHR17630:SF32 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGCCTGCAGGGCAGACGTATGGCAACAGGCAGAGATCGAACAGCGGG |
| Internal bar code: | TAGTCTCTATTATAATAAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3778 |
| LEAP-Seq percent confirming: | 82.8571 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGACTCGGATCGCATCAT |
| Suggested primer 2: | GTTTCATCATCAACCGGCGG |