| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037507 |
| Chromosome: | chromosome 6 |
| Location: | 4964032 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281350 | LON1 | (1 of 1) K08675 - Lon-like ATP-dependent protease [EC:3.4.21.-] (PRSS15, PIM1); Mitochondrial LON protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAAGGAGGGAACGGCTGGGGGGGGGGAGGGGCTGGGTGTGGCCCCTG |
| Internal bar code: | GTTGGCCTCTGGTAAGTTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATTCGGTCTGAGGAGCT |
| Suggested primer 2: | GGTGGTGGTATGGGTGAAGG |