| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037518 |
| Chromosome: | chromosome 10 |
| Location: | 445212 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420750 | RPL30 | Cytosolic 80S ribosomal protein L30; (1 of 1) K02908 - large subunit ribosomal protein L30e (RP-L30e, RPL30) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGCCATCCAGGGCCATCAGGTGCTCGTACTCCCCCCGCTGTGCTGCC |
| Internal bar code: | AAAACTCGCGTCAGCCCACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGCTATGCTCGCCAAGA |
| Suggested primer 2: | GGCTCTGGCTGCAAAATAGC |