| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.037538 |
| Chromosome: | chromosome 10 |
| Location: | 2677127 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437400 | (1 of 12) 2.7.11.18 - [Myosin light-chain] kinase / Smooth-muscle-myosin-light-chain kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACCATTCGGATGGCCCATCTGGCCCCCGCATGAACTTCACCGGTAAGT |
| Internal bar code: | GGCTACGTTGTCGTCCTTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3376 |
| LEAP-Seq percent confirming: | 94.5312 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 128 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGGGCTCTTGCTAGGCT |
| Suggested primer 2: | TACGGGTCAGTGTGCACTTC |