| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037543 |
| Chromosome: | chromosome 16 |
| Location: | 1733188 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654850 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCCTGGCCGGAAGACGTCCGTCACCTTTCTCGACACATAGTTTGAAT |
| Internal bar code: | TCCGAAAGCTTAATGGGTCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4625 |
| LEAP-Seq percent confirming: | 85.8696 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCTCAACTCATCAACCG |
| Suggested primer 2: | ATGACTGGTTGCCTGGGATG |