| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037553 |
| Chromosome: | chromosome 9 |
| Location: | 4452415 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400664 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCAGTACGGGGACGGCAACGGTGACGGCGCGACGGCGGCGGCGCCGG |
| Internal bar code: | GATCTTGAAAGAGCATTAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5247 |
| LEAP-Seq percent confirming: | 96.3636 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 165 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCATCCGACTGCTAG |
| Suggested primer 2: | AGGAGGCGATTGAGCTCATG |