| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037554 |
| Chromosome: | chromosome 7 |
| Location: | 2101228 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g326250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGGTGTTACGTTACTTCACCATGTACCGCACACGCCCCATACCTAGG |
| Internal bar code: | ATGCTCAATCGCTCATCGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1036 |
| LEAP-Seq percent confirming: | 62.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTCGGATGACAGCGTCC |
| Suggested primer 2: | GCGAATGCCTGCATGTACTG |