| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.037556 |
| Chromosome: | chromosome 1 |
| Location: | 5491251 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038900 | (1 of 5) PTHR11614:SF87 - MONOGLYCERIDE LIPASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACTATCTGCCGGAGGACGCCATCCACTTTCTGGAGGAGGTGCTGCAG |
| Internal bar code: | TTCACGGGTGAATCCTTCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGAACTCACTGTTTCCGA |
| Suggested primer 2: | CCGCAGTAGCAGATCTCCAG |