Insertion junction: CLIP2.037566_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.037566
Chromosome:chromosome 6
Location:930316
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256200 HAT3 Histone acetyltransferase 3; (1 of 1) K06062 - histone acetyltransferase (PCAF, KAT2, GCN5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGAATGCCTGCTCGTGGAAGGCGCGGTAGGTGATGCCGCCGATGACG
Internal bar code:GAACGAAAAAAGCGCGAGTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:486
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGACATCGCATTCCGCTA
Suggested primer 2:TCACTGAACCGTCACAAGCA