| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037571 |
| Chromosome: | chromosome 14 |
| Location: | 1944737 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801623 | (1 of 1) IPR006119 - Resolvase, N-terminal catalytic domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCCTCAAAAGCCTTCAGTTGCTCCTTGCGCATCTCACACCCTACCCGG |
| Internal bar code: | TGATTCAACAGGTGTCACGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2866 |
| LEAP-Seq percent confirming: | 57.3913 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 115 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCGATCTCGCCACAACA |
| Suggested primer 2: | CACAAACAAAGCGTGGGAGG |