| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037600 |
| Chromosome: | chromosome 1 |
| Location: | 1493331 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008000 | (1 of 1) K18666 - activating signal cointegrator complex subunit 1 (ASCC1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGTCCCATCCCTACGCCCCAGCTCCAACACTTCCACATTTTCCTTTG |
| Internal bar code: | GACACGACCGAGCGGAGATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACCCGAAACCATGTGCC |
| Suggested primer 2: | CTCTCTGACCCGTTGCAACT |