| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037601 |
| Chromosome: | chromosome 17 |
| Location: | 4385394 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730550 | (1 of 3) PTHR22298:SF39 - ENDOGLUCANASE 7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACACACACACACACACACACACACACACACACACACACACACA |
| Internal bar code: | ATTCCCCACCTTGGCAGTGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 732 |
| LEAP-Seq percent confirming: | 73.5294 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGGTGTTGCTTGGTTG |
| Suggested primer 2: | CGGGTGAGAAGTGACAACGA |