Insertion junction: CLIP2.037606_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.037606
Chromosome:chromosome 14
Location:2675323
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g625625 FTSH11 FtsH-like membrane ATPase/metalloprotease; (1 of 2) IPR000642//IPR003593//IPR005936//IPR011704//IPR027417 - Peptidase M41 // AAA+ ATPase domain // Peptidase, FtsH // ATPase, dynein-related, AAA domain // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATCCGCACCTTCAAGGACGTCAAGGGCTGTGACGAGGCCAAGGAGGA
Internal bar code:ACCACCTCTTGGCAAGGTCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:591
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTACCGTGTTTCTGCTCT
Suggested primer 2:CCGTTCCCGTCTAGCTCATC