Insertion junction: CLIP2.037621_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.037621
Chromosome:chromosome 2
Location:699122
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800169 (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGCACGCACACACTCCTTCATAATCGTGTGCGCACACGCACTCCAG
Internal bar code:TACTCAAGGCATTGTACCAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:353
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTTCTAGCATGGCCTGGG
Suggested primer 2:GCTTGCTTGGGTTTGAGTGG