Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.037635 |
Chromosome: | chromosome 16 |
Location: | 5511137 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g682350 | (1 of 1) PTHR11685:SF154 - E3 UBIQUITIN-PROTEIN LIGASE ARI5-RELATED | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCATGTTCTCGCTCTCCGCGCTGTTCTTGGTGAGCCACGTCTTCACCG |
Internal bar code: | GGCGGGCGGCGCAGCAGACGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2878 |
LEAP-Seq percent confirming: | 89.1892 |
LEAP-Seq n confirming: | 66 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCCGGTGAGTTAATGGTGA |
Suggested primer 2: | TCCCATCACATTCAGCGCTT |