| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037657 |
| Chromosome: | chromosome 16 |
| Location: | 413047 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693601 | ISG-C2,ISG2 | (1 of 5) PTHR16631//PTHR16631:SF9 - FAMILY NOT NAMED // GLUCAN 1,3-BETA-GLUCOSIDASE; Hydroxyproline-rich cell wall protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCGGATCTCAGCATGGCGACGGGCTACGTGATTCCCTCGAACAAGGT |
| Internal bar code: | CAGGCCATGATCATCCTGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 67.6471 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAGGAGAGTGTTGGGTG |
| Suggested primer 2: | ATCGCTAGAGTGCTCGTTCG |