| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037659 |
| Chromosome: | chromosome 16 |
| Location: | 7420327 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680902 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACGACTGCGTGCCGTGGTTGCGACTTTGAAGACTGTGTGTTGAGCT |
| Internal bar code: | TTAGCCCTATCGTAATCAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3297 |
| LEAP-Seq percent confirming: | 67.8571 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGCGTGGTTAATCATGC |
| Suggested primer 2: | GGACAGCGTTGGGGAAGTTA |