Insertion junction: CLIP2.037680_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.037680
Chromosome:chromosome 2
Location:6884345
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388726 (1 of 1) 3.1.3.32 - Polynucleotide 3'-phosphatase / 2'(3')-polynucleotidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTCGGAGAAGTCGTCCACTTCGTCGTCATACTTCTTGCTCCGCTTCGG
Internal bar code:GCAGGTGCTGGTGGAGAACATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2378
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TAAATGGTGGTCACTGCGCT
Suggested primer 2:ACATAACACCTCCAAGGCGG