| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037687 |
| Chromosome: | chromosome 4 |
| Location: | 3482657 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227850 | (1 of 1) IPR004192//IPR028978 - Ubiquinol cytochrome reductase, transmembrane domain // Chorismate pyruvate-lyase/UbiC transcription regulator-associated domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGGCTCAGGCTGACCAGGTTGGTGAGGCGCGCACACAGCGGGTACAG |
| Internal bar code: | TGGCATGGACTCATTGGAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1645 |
| LEAP-Seq percent confirming: | 69.1176 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACAGGT |
| Suggested primer 2: | AGGAGGAATGCGCATATGGG |