| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.037700 |
| Chromosome: | chromosome 5 |
| Location: | 640130 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245150 | (1 of 30) PF13450 - NAD(P)-binding Rossmann-like domain (NAD_binding_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGAGCGGCAAAGCCTCAAAGGGGAGGGGGGTGCCGTACAGCACGGGC |
| Internal bar code: | GCTGTCGACCAAATATATGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2251 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACCACGGGTGTCAGTTC |
| Suggested primer 2: | CTCCCTTCCCACATTCCCAC |