| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.037720 |
| Chromosome: | chromosome 6 |
| Location: | 1236540 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258583 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTGCACGACGATATCTGGCTTTGTCTGCGTGCGGCACAACGGGTGAA |
| Internal bar code: | AAGTACCGTACCCTCTGCCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1592 |
| LEAP-Seq percent confirming: | 53.5714 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAAGCAGAGGGCCAGAA |
| Suggested primer 2: | TTCTTGGGGCTTGTCGACTC |