| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037726 |
| Chromosome: | chromosome 10 |
| Location: | 4694061 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGCGCGCTGGGCGCCAGGCTAAGGTCGGGGTCCAGCAGCATGCGCAG |
| Internal bar code: | CTACGTCGATTCTGCTCTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 994 |
| LEAP-Seq percent confirming: | 39.1304 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGATTTGCTGGTGCCG |
| Suggested primer 2: | AAAGTCCTCAACCGCGACTT |