| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037726 |
| Chromosome: | chromosome 10 |
| Location: | 4694062 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGCAGCGCCTGCGGGTGCGGTCGTGCGGGCGGGCGGGCGCGTGCGAG |
| Internal bar code: | ATGGTTGTGGGCCGCATGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTCCTCAACCGCGACTT |
| Suggested primer 2: | TGTTTGATTTGCTGGTGCCG |